f actin polymerization buffer (Cytoskeleton Inc)

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Cytoskeleton Inc f actin polymerization buffer
F Actin Polymerization Buffer, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+buffer/Actin+polymerization+buffer/bio_rxiv__64898__2026__05__06__723191-322-6-9
Average 95 stars, based on 76 article reviews

f actin polymerization buffer - by Bioz Stars, 2026-09

95/100 stars

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Article Title: Training Generalized Segmentation Networks with Real and Synthetic Cryo-ET data
Article Snippet: Stock actin (10 mg/mL) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc. (Cat # BSA01-001, 5mM Tris-HCl pH 8.0, 0.2 mM CaCl , and 0.2 mM ATP), and left on ice for 30 min. Next, 10 uL of actin polymerization buffer (Cat # BSA02-001, 50 mM KCl, 20 mM MgCl , and 1 mM ATP) was added, mixed well, and placed at RT for one hour to polymerize.

Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging.
Article Snippet: Actin ligand 17-actin (1 mM in DMSO) was added to either supplemented actin buffer alone or in the presence of 0.4 mg ml−1 G-actin (catalogue no. AKL99, Cytoskeleton).

Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
Article Snippet: The supplemented actin buffer contained 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl 2 (from General Actin Buffer, catalogue no. BSA01, Cytoskeleton), 50 mM KCl, 2 mM MgCl 2 , 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, catalogue no. BSA02, and ATP, catalogue no. BSA04, Cytoskeleton).

Article Title: Rapid Synthesis of Cryo-ET Data for Training Deep Learning Models
Article Snippet: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc. (Cat # BSA01–001, 5mM Tris-HCl pH 8.0, 0.2 mM CaCl 2, and 0.2 mM ATP), and left on ice for 30 min. Next, 10 ul of actin polymerization buffer (Cat # BSA02–001, 50 mM KCl, 20 mM MgCl 2 , and 1 mM ATP) was added, mixed well, and placed at RT for one hour to polymerize.

Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
Article Snippet: Actin ligand 17 -actin (1 mM in DMSO) was added to either supplemented actin buffer alone or in the presence of 0.4 mg ml −1 G-actin (catalogue no. AKL99, Cytoskeleton).

Concentration Assay:

Article Title: Measurement and Characterization of the Electrical Properties of Actin Filaments
Article Snippet: In total, 1 mg of unlabeled lyophilized actin powder (cytoskeleton actin protein (>99% pure): rabbit skeletal muscle Cat. # AKL99) was reconstituted in 0.333 mL deionized water (DI-H 2 O). .. Then, it was diluted by adding 2.165 mL of general actin buffer (cytoskeleton, general actin buffer Cat. # BSA01) and 5 μL of ATP (cytoskeleton, adenosine 5′-triphosphate disodium salt (ATP) Cat. # BSA04, 100 mM stock) was added to yield a final ATP concentration of 0.2 mM. ..

Produced:

Article Title: AKAP2-anchored extracellular signal-regulated kinase 1 (ERK1) regulates cardiac myofibroblast migration.
Article Snippet: .. Filamentous Actin (F-actin) was produced as it follows: non-muscle actin (Cytoskeleton Inc., catalog n◦ APHL99) was resuspended in 250 μl of general actin buffer (Cytoskeleton Inc., catalog n◦ BSA01-001) supplemented with 0,2 mM ATP (Cytoskeleton Inc., catalog n◦ BSA04001) for 1 h on ice. .. 25 μl of actin polymerization buffer (Cytoskeleton Inc., catalog n◦ BSA02-001) were added to the depolymerized actin protein prepared as indicated above and incubated for 1 h at 24 ◦C.

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